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Image Search Results
Journal: Inflammatory Bowel Diseases
Article Title: Microbial Disruption of Autophagy Alters Expression of the RISC Component AGO2, a Critical Regulator of the miRNA Silencing Pathway
doi: 10.1097/mib.0000000000000553
Figure Lengend Snippet: FIGURE 2. Disruption of autophagy increases AGO2 in MDAMC cells. A, Western blotting for AGO2 and LC3 in lysates obtained from MDAMC cells treated with control siRNA, ATG12 siRNA, or VacA. B, Graph depicts quantification of AGO2 relative to b-actin levels (n ¼ 3 experiments **P , 0.01, analysis of variance with Dunnett’s post hoc test). C, Representative confocal image of LC3-GFP expressing control siRNA or ATG12 siRNA cells, in the presence or absence of VacA. LC3 is depicted in green, AGO2 is depicted in red, and p62 is depicted in blue.
Article Snippet: Proteins were then transferred onto a nitrocellulose membrane that was blocked with 5% milk in Tris-buffered saline with tween and probed with antibodies diluted in 5% milk in Tris-buffered saline with tween: 1:500 AGO2 rabbit polyclonal antibody (Abcam), 1:2000
Techniques: Disruption, Western Blot, Control, Expressing
Journal: Inflammatory Bowel Diseases
Article Title: Microbial Disruption of Autophagy Alters Expression of the RISC Component AGO2, a Critical Regulator of the miRNA Silencing Pathway
doi: 10.1097/mib.0000000000000553
Figure Lengend Snippet: FIGURE 1. Autophagy-deficient MEF cells display increased AGO2. A, Representative Western blot for AGO2 and LC3 in lysates obtained from Atg52/2 or WT cells. B, Representative confocal image of ATG52/2 or WT LC3-GFP expressing cells. LC3 is depicted in green. AGO2 is depicted in red. C, Graph depicts quantification of AGO2 relative to b-actin levels (n ¼ 5 experiments; *P , 0.05). D, Representative Western blot for AGO2 and LC3 in lysates obtained from Atg52/2 or WT cells treated with bafilomycin to disrupt autophagy.
Article Snippet: Proteins were then transferred onto a nitrocellulose membrane that was blocked with 5% milk in Tris-buffered saline with tween and probed with antibodies diluted in 5% milk in Tris-buffered saline with tween: 1:500 AGO2 rabbit polyclonal antibody (Abcam), 1:2000
Techniques: Western Blot, Expressing